ty1 tag antibody Search Results


93
Rockland Immunochemicals mouse anti ty antibodies
Mouse Anti Ty Antibodies, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ty1+tag+antibody/pmc05101828-242-73-78?v=Rockland+Immunochemicals
Average 93 stars, based on 1 article reviews
mouse anti ty antibodies - by Bioz Stars, 2026-07
93/100 stars
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90
DIAGENODE DIAGNOSTICS ty1 tag antibody
Ty1 Tag Antibody, supplied by DIAGENODE DIAGNOSTICS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ty1+tag+antibody/pmc10146891__sciadv__ade8184_sm-48-13-16?v=DIAGENODE+DIAGNOSTICS
Average 90 stars, based on 1 article reviews
ty1 tag antibody - by Bioz Stars, 2026-07
90/100 stars
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90
DIAGENODE DIAGNOSTICS bb2 against ty1 tag antibody
(A) Coimmunoprecipitation assays verify the interaction of PRAME with the EKC subunits OSGEP and LAGE3. Constructs expressing the indicated proteins were transiently transfected in 293T cells. Anti-TAG immunoprecipitations were performed with rabbit HA antibody (Abcam). TAG-PRAME was detected with monoclonal anti-HA (Covance) and TTE-tagged proteins with monoclonal <t>BB2</t> (Diagenode) which recognizes the <t>TY1</t> tag. The lower panel shows a scheme of the tagged proteins used (tags and coding sequences are not on scale). (B-C) PRAME directly interacts with EKC complex through OSGEP and LAGE3. SF21 cells were co-infected with baculoviruses expressing the indicated proteins. Anti-FLAG immunoprecipitations were performed, and total cell lysates and eluates were analyzed by western blotting. Panels showing anti-HA immunoblots are from the same exposure of the same blot, as are the panels showing anti-cMyc immunoblots. Asterisks indicate light chains of the antibodies used in the immunoprecipitation.
Bb2 Against Ty1 Tag Antibody, supplied by DIAGENODE DIAGNOSTICS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ty1+tag+antibody/pmc03418287-229-43-48?v=DIAGENODE+DIAGNOSTICS
Average 90 stars, based on 1 article reviews
bb2 against ty1 tag antibody - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier


N/A
Ty1 Tag Monoclonal Antibody for Western Blot ChIP
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(A) Coimmunoprecipitation assays verify the interaction of PRAME with the EKC subunits OSGEP and LAGE3. Constructs expressing the indicated proteins were transiently transfected in 293T cells. Anti-TAG immunoprecipitations were performed with rabbit HA antibody (Abcam). TAG-PRAME was detected with monoclonal anti-HA (Covance) and TTE-tagged proteins with monoclonal BB2 (Diagenode) which recognizes the TY1 tag. The lower panel shows a scheme of the tagged proteins used (tags and coding sequences are not on scale). (B-C) PRAME directly interacts with EKC complex through OSGEP and LAGE3. SF21 cells were co-infected with baculoviruses expressing the indicated proteins. Anti-FLAG immunoprecipitations were performed, and total cell lysates and eluates were analyzed by western blotting. Panels showing anti-HA immunoblots are from the same exposure of the same blot, as are the panels showing anti-cMyc immunoblots. Asterisks indicate light chains of the antibodies used in the immunoprecipitation.

Journal: PLoS ONE

Article Title: The Human EKC/KEOPS Complex Is Recruited to Cullin2 Ubiquitin Ligases by the Human Tumour Antigen PRAME

doi: 10.1371/journal.pone.0042822

Figure Lengend Snippet: (A) Coimmunoprecipitation assays verify the interaction of PRAME with the EKC subunits OSGEP and LAGE3. Constructs expressing the indicated proteins were transiently transfected in 293T cells. Anti-TAG immunoprecipitations were performed with rabbit HA antibody (Abcam). TAG-PRAME was detected with monoclonal anti-HA (Covance) and TTE-tagged proteins with monoclonal BB2 (Diagenode) which recognizes the TY1 tag. The lower panel shows a scheme of the tagged proteins used (tags and coding sequences are not on scale). (B-C) PRAME directly interacts with EKC complex through OSGEP and LAGE3. SF21 cells were co-infected with baculoviruses expressing the indicated proteins. Anti-FLAG immunoprecipitations were performed, and total cell lysates and eluates were analyzed by western blotting. Panels showing anti-HA immunoblots are from the same exposure of the same blot, as are the panels showing anti-cMyc immunoblots. Asterisks indicate light chains of the antibodies used in the immunoprecipitation.

Article Snippet: Commercial antibodies used were: mouse monoclonal FLAG-M2 (Sigma), c-Myc monoclonal (Roche Applied Science), rabbit HA (Bethyl Laboratories), mouse monoclonal HA.11 (Covance MMS-101P), rabbit Cul2 (Zymed Laboratories 51-1890), mouse ElonginC (BD Transduction Laboratories 610760), goat ElonginB (Santa Cruz P-16, sc-23407), mouse TPRKB (Abcam ab68245), mouse BB2 against TY1 tag (Diagenode), GFP (Santa Cruz).

Techniques: Construct, Expressing, Transfection, Infection, Western Blot, Immunoprecipitation

ChIP-qPCR experiments using BB2 antibody were performed on K562 cells lines stably expressing TTE-OSGEP or TTE-LAGE3. As control for specificity, ChIPs were performed on the parental cells (wt) and on cells transduced with the vector expressing the tag only (K562-TTE) using a panel of primer sets for PRAME biding sites. Values are expressed as mean recoveries ± standard deviation from three independent experiments.

Journal: PLoS ONE

Article Title: The Human EKC/KEOPS Complex Is Recruited to Cullin2 Ubiquitin Ligases by the Human Tumour Antigen PRAME

doi: 10.1371/journal.pone.0042822

Figure Lengend Snippet: ChIP-qPCR experiments using BB2 antibody were performed on K562 cells lines stably expressing TTE-OSGEP or TTE-LAGE3. As control for specificity, ChIPs were performed on the parental cells (wt) and on cells transduced with the vector expressing the tag only (K562-TTE) using a panel of primer sets for PRAME biding sites. Values are expressed as mean recoveries ± standard deviation from three independent experiments.

Article Snippet: Commercial antibodies used were: mouse monoclonal FLAG-M2 (Sigma), c-Myc monoclonal (Roche Applied Science), rabbit HA (Bethyl Laboratories), mouse monoclonal HA.11 (Covance MMS-101P), rabbit Cul2 (Zymed Laboratories 51-1890), mouse ElonginC (BD Transduction Laboratories 610760), goat ElonginB (Santa Cruz P-16, sc-23407), mouse TPRKB (Abcam ab68245), mouse BB2 against TY1 tag (Diagenode), GFP (Santa Cruz).

Techniques: ChIP-qPCR, Stable Transfection, Expressing, Control, Transduction, Plasmid Preparation, Standard Deviation